Laboratory Design We all know there are many, so how many people know about PCR laboratory design? And PCR laboratory What are the basic requirements for design?

1, PCR laboratory design plane layout;

In principle, the PCR amplification testing laboratory is divided into four separate working areas: reagent storage and preparation area, specimen preparation area, amplification reaction mixture preparation and amplification area, and amplification product analysis area. In order to avoid cross-contamination, entering each work area strictly follows a single direction, that is, only from the reagent storage and preparation area → specimen preparation area → amplification reaction mixture preparation and amplification area → amplification product analysis area. The transfer of reagents and samples between each experimental area should be carried out through the transfer window.

2. Laboratory air conditioning and ventilation system design and pressure control;

The PCR laboratory does not have strict purification requirements, but in order to avoid the possibility of cross-contamination between various experimental areas, it is advisable to adopt a full-supplied full-exhaust air organization form. At the same time, the ratio of air supply and exhaust must be strictly controlled to ensure the pressure requirements of each experimental area.

2.1. Specimen preparation area;

The main operations in this area are sample preservation, nucleic acid (RNA, DNA) extraction, storage, and adding to the amplification reaction tube and measuring DNA synthesis. The pressure gradient in this area is required to be positive pressure relative to the adjacent area to avoid aerosol pollution entering the area from the adjacent area. In addition, since contamination caused by aerosols may occur during the sample addition operation, unnecessary walking in the area should be avoided.

2.2. Reagent storage and preparation area;

The main operations in this experimental area are the preparation of storage reagents, the dispensing of reagents and the preparation of the main reaction mixture. Reagents and materials used for sample preparation should be transported directly to this area and not through other areas. The reagent raw materials are stored in this zone and prepared into the required storage reagents in this zone. There are no strict requirements for the control of airflow pressure in this area.

2.3. Amplification product analysis area;

The main operation in this area is the determination of amplified fragments. This area is the main source of contamination of amplified products, so the pressure gradient in this area is required to be negative pressure relative to the adjacent area to avoid the diffusion of amplified products from this area to other areas.

2.4. Preparation of amplification reaction mixture and amplification area;

The main operation in this area is DNA amplification. In addition, the addition of the prepared DNA template (from the sample preparation area) and the preparation of the main reaction mixture (from the reagent storage and preparation area) into a reaction mixture can also be carried out in this area. The pressure gradient in this area is required to be negative pressure relative to the adjacent area to avoid aerosol leakage from this area. In order to avoid pollution caused by aerosols, unnecessary walking in the area should be minimized. Individual operations such as sample addition should be carried out in the ultra-clean bench.

3.1. Strict division of work area;

(1) The setting of each experimental area is reasonable;

(2) Each experimental area must have obvious marks (such as eye-catching house numbers or different ground colors, etc.) to avoid confusion of equipment, items, reagents, etc. in different experimental areas.

3.2. Reasonable system settings;

(1) Reasonable air conditioning and ventilation system settings, try to use full delivery and full exhaust air conditioning systems;

(2) Strict airflow pressure control to ensure different pressure requirements in different experimental areas.

3.3. Standard operation;

(1) The technicians of the amplification test laboratory shall be trained on the job, and only after the training is qualified can they be engaged in the work of clinical gene amplification test;

(2) During the experimental operation, the operator must wear gloves and change them frequently. In addition, the use of disposable caps in operation is also an effective measure to prevent pollution;

(3) The cleaning work is timely and correct. After the experimental work is over, clean the area immediately. In addition to the conventional disinfection liquid to wipe and disinfect the surface or ultraviolet light irradiation disinfection, some experimental equipment should also be autoclaved.

3.4. Strict management;

(1) Strictly control personnel entering and leaving the laboratory. Personnel who are not related to the experiment are not allowed to enter and leave the laboratory at will. If possible, independent passages and doors for entering and leaving the entire experimental area shall be set;

(2) Minimize unnecessary walking in the experimental area to reduce the possibility of cross-contamination.

(3) The amplification product analysis area is the main source of amplification product contamination. The waste liquid cannot be dumped in the laboratory. After soaking and disinfecting in the disinfectant, discard it in a place away from the laboratory, and wait for the used tip once. Sexual materials should also be treated uniformly after soaking and disinfecting with disinfectant, such as incineration;

(4) Certain genetic mutations and toxic substances may be used in the amplification product analysis area, so special attention should be paid to the safety protection of experimental personnel.

3.5. Complete laboratory supporting facilities;

Complete laboratory supporting facilities are a necessary condition to ensure experimental work. Corresponding equipment and instruments, such as ultra-clean workbenches, centrifuges, samplers, etc., should be equipped according to the different experimental contents of each laboratory.